Fig. 4.

IL-6 is critical for EC-induced macrophage alternative activation. a, b Mouse microvascular brain ECs were pretreated with the glioma-CM for 24 h. Mouse BM-derived macrophages were co-cultured with pretreated ECs for 5 days in the presence of control IgG, anti-CSF-1 antibody, or anti-IL-6 antibody or both antibodies. The cells were stained with anti-CD11b, anti-CD86, anti-CD206 antibodies, and analyzed by flow cytometry. a Representative sorting for CD206 expression in CD11b⁺ cells. b Quantified data in sorted CD11b⁺ cells (n = 3–5, mean ± SEM). c–e Mouse BM-derived macrophages were treated with IL-6 and CSF-1 for 5 days. c, d The cells were stained with anti-CD11b, anti-CD86, and anti-CD206 antibodies, and analyzed by flow cytometry. c Representative sorting for CD206 and CD86 expression in CD11b⁺ cells. d quantified data in sorted CD11b⁺ cells (n = 3, mean ± SEM). e Cell lysates were immunoblotted