Fig. 3.
Validation on synthetic data. Short synthetic reads of recombined TRB sequences were generated with known recombination statistics, and given to IGoR as input to reinfer these statistics. Inference with 105 TRB sequences and a typical sequencing error rate of 10−3 gives excellent agreement for a gene usage and insertion statistics and b deletion statistics (Pearson’s r for deletions is calculated on the joint statistics of gene usage and deletion number; cross size scales with gene usage). c Discrepancy between true and inferred values of the recombination statistics for TRB, measured by the Kullback–Leibler divergence, as a function of the number of unique sequences in the sample, and decomposed according to the features of the recombination scenario. d Same as c, for increasing rates of sequencing errors