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. 2018 Feb 8;8:2630. doi: 10.1038/s41598-018-20815-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kinetics of uptake of CB, AF405 and Fl-MTX in A431 cells overexpressing OATP1B1, 1B3 or 2B1. Transport was measured in 96-well plates. Cells were incubated with increasing concentrations of Fl-MTX, CB or AF405 in the linear phase of uptake (2.5 minutes for Fl-MTX, 10 minutes (1B1, 2B1) or 15 minutes (1B3) for CB, and 15 minutes for AF405). Transport capacity was calculated based on calibration with known amounts of the dye. Uptake in A431-OATP cells without background signal (fluorescence in A431-mock cells) is shown. Data points indicate average ± SD values obtained in three independent experiments.