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. 2018 Feb 8;8:2644. doi: 10.1038/s41598-018-20781-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Electron microscopy of wild-type cells, and lipid droplet formation after chronic heat stress. (a) Wild-type cells at 25 °C. (b) Wild-type cells grown at 40.5 °C for 6 h. (c–e) Various types of vacuolar invaginations in cells grown at 40.5 °C for 6 h. Scale bar; 2 μm for (a) and (b), and 0.5 μm for (c–e). Black and white arrows indicate vacuoles and MVBs, respectively. (f) Number of lipid droplets per a cell at 25 °C and 40.5 °C for 6 h. Average were calculated from EM images of fifty-two cells that were randomly taken. Error bar indicates SE, and significant difference was calculate using Student’s-t test.