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. 2018 Feb 8;8:2656. doi: 10.1038/s41598-018-21060-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Results for HDAC assay. (A) deacetylation (left), debutyrylation (middle), and demalonylation (right) efficiencies on H3K9/14/18/23/27/36/37 and H4K5/8/12/16 of Sirt1 (24 ng/µL). (B) Those of Sirt2 (24 ng/µL). (C) Those of Sirt3 (12 ng/µL). (D) Those of Sirt5 (2 ng/µL). (E) Those of Sirt6 (12 ng/µL). (F) Those of Sirt7 (12 ng/µL). The average and SD (error bars) obtained from n = 3 independent HDAC assays are indicated, where measurements were conducted in duplicate for each HDAC assay.