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. 2018 Feb 8;8:2660. doi: 10.1038/s41598-018-20999-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Recruitment of RNAPII and acetylated H3K9 on transcription initiation sites of MMP genes in human keratinocytes. HKerE6SFM cells were exposed to 80 mJ/cm² of UVB irradiation or mock treated. Chromatin was prepared from each sample 24 hours after the treatment. Antibodies against RNAPII, H3 and acetylated H3K9 were used for the immunoprecipitation. Precipitated chromatin samples were analysed by qPCR. Enrichments of RNAPII, H3 and acetylated H3K9 were calculated as % of total inputs and normalized to the H3 content of control samples. The means, standard deviations and significance levels based on three independent experimental triplicates are indicated. Star indicates statistical significance between the dataset (paired Anova *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). (A) RNAPII and H3K9ac enrichments in transcription initiation sites of MMP genes encoded by the 11q22.3 genomic region. (B) RNAPII and H3K9ac enrichments of MMP9 negative control gene.