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. 2018 Feb 8;9:571. doi: 10.1038/s41467-018-03013-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — SK11 and SK12 interact with TTG1. a GST pull-down assay of the interaction of TTG1 with SK11 and SK12. GST, GST-SK11, and GST-SK12 were used as baits, and their loading amounts stained with Ponceau S are shown in the bottom panel. The input of prey proteins, MBP and MBP-TTG1, and their corresponding pull-downed signals are shown in the upper panel. b BiFC analysis of the interaction between TTG1 and SK11/SK12 in tobacco epidermal cells. Scale bar, 200 μm. c Yeast two-hybrid assay of the interaction between TTG1 and SK11/SK12. Ten-fold serial dilutions of transformed yeast cells were grown on SD-Trp/-Leu medium (upper panel) and SD-Trp/-Leu/-His medium supplemented with 0.3 mM 3-amino-1, 2, 4-triazole (3AT) (lower panel). d In vivo interaction between 4HA-TTG1 and SK11^E292K-GFP in Arabidopsis siliques shown by co-immunoprecipitation (CoIP). Total protein extracts from siliques of SK11:SK11^E292K-GFP in wild-type (WT) or g4HA-TTG1 background 4 days after pollination (DAP) were immunoprecipitated by anti-HA antibody. The input and co-immunoprecipitated proteins were subjected to immunoblot analysis with anti-HA (upper panel) or anti-GFP antibody (lower panel). Uncropped original scans of immunoblots are shown in Supplementary Fig. 17