Fig. 6.

Effect of S4–S5 linker interruptions on Kv11.1 ionic current mode shift. a Representative ion currents from continuous wild-type (WT) and splits 545 and 539 studied with the voltage protocols shown at − 80/− 100 (top) and + 40 mV (bottom) holding potentials. b Averaged I versus V relationships from currents measured at the end of the variable depolarization step at different voltages as in panel a. Open and closed symbols correspond to data obtained in the same oocytes at hyperpolarized and depolarized holding potentials, respectively. c Plots of normalized peak tail current magnitudes as a function of depolarizing voltage for channels split at different positions along the S4–S5 linker. Data from continuous WT channels are also shown for comparison. Averaged values from four to eight cells are shown. Sometimes, error bars are smaller than the symbols. Continuous lines are Boltzmann fits to the data as indicated in “Materials and methods” section. Hyperpolarized (open symbols) and depolarized (closed symbols) holding potential values are indicated in the graphs