FIG. 3.

G6PDH activity regulates the amplitude of circadian rhythms in mouse fibroblasts. Western blot analysis (A, B) and its quantification (C, D) from cells silenced for Gpi or G6pdh (mean ± SEM, Student's t-test). (E) Effect of RNAi silencing of Gpi and G6pdh on PER2::LUC rhythms (mean ± SEM). (F) Silencing of G6PDH but not of GPI results in decreased clock protein amplitude, measured at the second peak of bioluminescence (mean ± SEM, Student's t-test). *p < 0.05; **p < 0.01, ns, p > 0.05. G6PDH, glucose-6-phosphate dehydrogenase; GPI, phosphoglucose isomerase.