Figure 1.

Akt3 overexpression provokes salivary gland tumorigenesis: (A) Scheme depicting breeding strategy of MMTV‐driven expression of HA‐tagged Akt3 via tTA. Dox administration represses transgenic Akt3 expression. (B) Expression of the Akt3 transgene in salivary gland tissues of Dt animals was verified by qRT‐PCR (n = 5) analysis and (C) by western blot analysis. (D) Kaplan–Meier plot depicting time until tumors were clearly visible in Ctrl and Dt mice (n > 10 mice per group). (E) IHC of salivary glands of Ctrl and Dt mice probed for HA expression. Pictures on the right show a higher magnification of the same sections.