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. 2017 Dec 26;7(2):380–396. doi: 10.1002/cam4.1276

Figure 3.

Figure 3

SM1044‐induced apoptosis is dependent on the generation of autophagy. (A) SU‐DHL‐4, SU‐DHL‐10, and OCI‐LY3 cells were pretreated with CQ or Baf A1 for 1 h, followed by SM1044 treatment for another 24 h. The respective expressions of caspase‐8, ‐9, ‐3, and PARP were detected by western blot. (B) Apoptosis was measured by flow cytometry (mean ± SEM, n = 3). SU‐DHL‐4: SM1044 versus control, P < 0.001; SM1044 versus CQ plus SM1044, P < 0.001; SM1044 versus Baf A1, P < 0.001. SU‐DHL‐10: SM1044 versus control, P < 0.001; SM1044 versus CQ plus SM1044, P = 0.0077; SM1044 versus Baf A1, P < 0.001. OCI‐LY3: SM1044 versus control, P < 0.001; SM1044 versus Baf A1, P = 0.041. (C) SU‐DHL‐4 cells were stably transfected with scramble or LC3 shRNA, then treated with SM1044 for 24 h. Apoptosis was measured by flow cytometry (mean ± SEM, n = 3). Scramble + SM1044 versus sh‐LC3 + SM1044, P = 0.0012. (D) The respective expressions of caspase‐8, ‐9, ‐3, and PARP were detected by western blot in the stably transfected cells after SM1044 treatment for 24 h. *P < 0.05, **P < 0.01, ***P < 0.001.