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SALL4 enhances stemness and modulates the expression of CD44 variant exons. (A) Spheres of the control and SALL4 knockdown cells are shown. SUM159 and Hs578T cells were used. Arrowheads indicate spheres larger than 50 μm. (B) Numbers of spheres are graphed. (C) Quantification of the expression of CD44 variant exons and total CD44 is shown. Gene structure of CD44 is depicted. The graph shows relative mRNA levels to the shGFP control. Scale bar: 100 μm. *P < 0.05, **P < 0.01.
