Table 3.

PRP preparation protocol in included trials

First author [Ref.], year	Mode of preparation	Activators	Centrifugation	Time of centrifugation	Platelet enrichment (fold)	Blood volume	PRP volume
Anitua [29], 2017	Single spin method	PRGF activator (BTI Biotechnology Institute)	580 rpm	8 min	×2±0.3 mean	18 mL	3–4 mL
Alves [33], 2016	Single spin method	Calcium chloride (10%, 0.15 mL)	460 g	8 min	×3	18 mLa	3 mL
Gentile [34], 2015	a. Cascade-Selphyl-Esforax system b. PRL platelet-rich lipotransfert system	Ca2+	a. 1,100 g b. 1,200 rpm	a. 10 min b. 10 min	-	a. 18 mL b. 60 mL	a. 9 mL b. 20 mL
Cervelli [28], 2014	Cascade-Selphyl-Esforax Kit	Ca2+	1,100 g	10 min	-	18 mL	9 mL
Singhal [12], 2015	Double spin method	Calcium chloride (9:1 ratio)	a. 1,500 rpm b. 2,500 rpm	a. 6 min b. 15 min	-	20 mL	8–12 mL
Gupta [32], 2017	Double spin method	-	-	-	-	-	-
Schiavone [36], 2014	GPS III Platelet Separation System a. Single spin at baseline b. Double spin at 3 months	No (Scalproller used to favor platelet activation (see Table III))	-	-	a. ×6–7 (×3.5–4 with addition of plasmatic protein concentrate) b. ×4 with addition of plasmatic protein concentrate	a. 60 mL b. 40 mL	a. 6–8 mL PRP + 3–4 mL of plasmatic protein concentrate = 9–12 mL; 0.2–0.3 mL per injection b. Same as above
Gkini [40], 2014	RegenA-PRPCentri (Regenlab) Single spin method	Calcium gluconate (0.1 mL per 0.9 mL of PRP; 1:9 ratio)	1,500 g	5 min	×5.8	16 mL	6 mL (0.05–0.1 mL/cm2)
Khatu [35], 2014	Manual Double Spin	Calcium chloride (1:9 ratio)	a. 1,500 rpm b. 2,500 rpm	a. 6 min b. 15 min	-	20 mL	2–3 mL
Takikawa [37], 2011	Manual Double Spin	-	a. 1,700 rpm b. 3,000 rpm	a. 15 min b. 5 min	×6	15 mL	3 mL
Puig [30], 2016	Angel PRP system (Cytomedix)	No	-	-	×2.75–3.4	60 mL	10 mL
Mapar [31], 2016	Double spin method using Tubex PRP tube (Moohan Enterprise)	Calcium gluconate (0.1 mL per mL of PRP)	a. 3,000 rpm b. 3,300 rpm	a. 6 min b. 3 min	×3	9 mLb	1.5 mL

^aPlus 2 mL of sodium citrate.

^bPlus 1 mL of acidic citrate dextrose.