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Steady-state kinetic parameters of HsFH, A308T, and H318Y.
| Enzyme | Vmax (µmole /min/mg) | kcat (s-1) | Km (mM) | kcat/Km (M-1 s-1) |
|---|---|---|---|---|
| HsFH | 170 ± 10 | 150 ± 9 | 1.9 ± 0.2 | 8 x 104 ± 1 x 104 |
| A308T | 0.18 ± 0.02 | 0.16 ± 0.02 | 1.1 ± 0.3 | 150 ± 20 |
| H318Y | 0.046 ± 0.003 | 0.041 ± 0.002 | 2.0 ± 0.2 | 21 ± 2 |
Values represent the mean of four independent replicates ± standard deviation.
