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Endogenous UCH-L1 is physically associated with LMP1. 293 cells were transfected with empty and LMP1-Flag expression vectors and were harvested 48 h later for LMP1 immunoprecipitation with anti-Flag-agarose beads. IPs and total lysates of proteins were separated in 4% to 20% PAGE. After transfer to a PVDF membrane, the blots were probed with the indicated antibodies. The results shown are representative of results of three independent experiments with similar outcomes.
