FIG 4 .

Neither C-terminal farnesylation nor deubiquitinating activity of UCH-L1 affects LMP1 presence in ectosome fractions of extracellular vesicles. The protein fractions obtained from sequential centrifugation at 20,000 × g (ectosomes) of the samples from the experiment represented in Fig. 3 were separated in 4% to 20% PAGE, and, after transfer to a PVDF membrane, the blots were exposed to the indicated antibodies. The results demonstrate that inhibition of cellular farnesylation with FTI-277 or UCH-L1-dependent deubiquitination with LDN-57444 (A), as well as expression of DUB-dead or farnesylation-impaired UCH-L1 mutants (B), did not change the levels of LMP1 in this fraction of extracellular vesicles.