The essential metabolic pathways of S. mutans UA159 for the initial library recovered on blood agar. Essential metabolic pathways are indicated by green lines. Shaded areas represent major metabolic processes (e.g., carbohydrate metabolism). We have also highlighted metabolic pathways that are essential (e.g., threonine biosynthesis). Abbreviations: TCA, tricarboxylic acid; CoA, coenzyme A; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LDH, lactate dehydrogenase; PEP, phosphoenolpyruvate; P, phosphate; BP, bisphosphate.