TABLE 1 .
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| P. aeruginosa PAO1 | ||
| MJ79 | Wild type | 14 |
| MJ80 | ΔgbdR | 15 |
| JM236 | ΔcdhR | This study |
| JM253 | Wild type attTn7::88-89intYFPCFP-2 | This study |
| JM339 | ΔcdhR attTn7::88-89intYFPCFP-2 | This study |
| JM340 | ΔgbdR attTn7::88-89intYFPCFP-2 | This study |
| MJ784 | ΔgbdR ΔcdhR | This study |
| P. aeruginosa PA14 | ||
| MJ101 | Wild type | 42 |
| MJ11 | ΔcdhR | 11 |
| MJ26 | ΔgbdR | 15 |
| MJ262 | ΔcdhCA | 11 |
| JM179 | ΔcdhR ΔgbdR | This study |
| E. coli | ||
| MJ340 | Wild-type S17λpir | |
| DH5α | NEB C2987 | NEB |
| T7Express | NEB C3016 | NEB |
| Plasmids | ||
| pMQ30 | Suicide vector, Gmr | 36 |
| pMQ80 | High-copy-no. Pseudomonas vector, Gmr | 36 |
| pMal-C2X | T7-expressing vector, MBP N-terminal tag, Ampr | NEB |
| pTNS2 | Plasmid carrying attTn7 transposase | 43 |
| pUC18-mini-Tn7T-Gm | Gmr on mini-Tn7T | 35 |
| pUC18-mini-Tn7T-Gm-eyfp | Gmr on mini-Tn7T with YFP | 35 |
| pUCP22 | High-copy-no. Pseudomonas stabilization vector, Gmr | 44 |
| pMW5 | lacZYA in pUCP22 | 32 |
| pMW79 | PA14 genomic clone of PA5380-PA5389 in pMQ80 | 11 |
| pPA5380KO | gbdR deletion construct in pEX18-Gm | 15 |
| pJAM22 | Promoter caiX-lacZYA transcriptional fusion A | This study |
| pJAM23 | Promoter caiX-lacZYA transcriptional fusion B | This study |
| pJAM24 | Promoter caiX-lacZYA transcriptional fusion C | This study |
| pJAM25 | Promoter caiX-lacZYA transcriptional fusion D | This study |
| pJAM50 | PA5389 in pMal-C2X | This study |
| pJAM76 | YFP-CFP in pMQ80 | This study |
| pJAM86 | CFP PA5388-PA5389 intergenic region YFP in pUC18mini, DR2 | This study |
| pJAM90 | PA5389 deletion construct in pMQ30 | This study |
| pJAM122 | Promoter caiX-lacZYA transcriptional fusion | This study |
| pJAM123 | Promoter caiX-lacZYA mut 1 transcriptional fusion | This study |
| pJAM124 | Promoter caiX-lacZYA mut 2 transcriptional fusion | This study |
| pJAM125 | Promoter caiX-lacZYA mut 3 transcriptional fusion | This study |
| pJAM126 | Promoter caiX-lacZYA mut 4 transcriptional fusion | This study |
| pJAM127 | Promoter caiX-lacZYA mut 5 transcriptional fusion | This study |
| pJAM130 | Promoter caiX-lacZYA mut 6 transcriptional fusion | This study |
| pJAM131 | C terminus of lacZ in pUCP22 | This study |
| pJAM135 | PcdhRlacZYA in pUCP22 | This study |