TABLE 2 .
Primers used in this study
| Primer | Sequence (5′ to 3′)a |
|---|---|
| Deletion constructs | |
| 5389GOIF1KpnI | ATAGGGTACCGAAGAACACCACCCACTGCT |
| 5389SOEGOIR1 | AAGTACGAAGGCGACTCGACCATGGAGAAGCCCATTACCGAGAAGC |
| 5389SOEGOIF1 | GCTTCTCGGTAATGGGCTTCTCCATGGTCGAGTCGCCTTCGTACTT |
| 5389GOIR1BamHI | ATCGTCTTCGCTGTTTTTCC |
| Protein expression construct | |
| 5389Mal-c2xF | GCATCAGAATTCTCCCAGGACTTCTGGTTTCT |
| 5389Mal-c2xR | GCATCAAAGCTTTCAGCCTCGCTCAGCTCGA |
| Primer extension | |
| 5388primerextension | 5′-Fluorescein 6-FAM-ACTGGCCAGGATCAGCAGG |
| 5389primerextension | 5′-Fluorescein 6-FAM-AGACAGTATCGGCCTCAGGAA |
| EMSA probes | |
| PA5388promF3 | AAGCTTGTGCCAGCGGTAGAGGTC |
| PA5388promR | TGAGGTACCTTGATTGTTTTTCTGCGAGGT |
| PA5388promRbiot | Biotin-TTGATTGTTTTTCTGCGAGGT |
| 5389EMSA-F | ATGAAAGCTTGCAGCAGGAGAAACCAGAAG |
| 5389EMSA-R-biot | Biotin-TTGATTGTTTTTCTGCGAGGT |
| 5389EMSA-Mut3F | GGACGGCGGCGAAGCGCACTGCGAAGACC |
| cbcXprom-F | CCGGCAAAGACCACTATGAT |
| cbcXprom-R-biot | Biotin-GAACTCCTCTGCAGGGTAAGG |
| dhcprom-F-biot | Biotin-GAGGCTTTCCTCCAGGCTCT |
| dhcprom-R | GGATGGTACCCTCTTCCGGCTCTTGTGATT |
| dhcprom-F | GAACTCCTCTGCAGGGTAAGG |
| Transcriptional reporters | |
| PA5388promR | TGAGGTACCTTGATTGTTTTTCTGCGAGGT |
| PA5388promF1 | ATGAAAGCTTACAGCAGGTCGCCTTTCTT |
| PA5388promF3 | AAGCTTGTGCCAGCGGTAGAGGTC |
| PA5388promF2 | ATGAAAGCTTGCAGCAGGAGAAACCAGAAG |
| PA5388promF4 | AAGCTTCTGCAGTGCAAGAGCTGGT |
| P5388pos | ATGAAAGCTTCGCTTGGCAATGGCCAGGTCGCT |
| P5388mut1 | ATGAAAGCTTCGCTTGGCAATGGCCAAATCGCT |
| P5388mut2 | ATGAAAGCTTCGCTTGGCAATGGCCAGAACGCT |
| P5388mut3 | ATGAAAGCTTCGCTTGGCAATGGCCAGGAAGCT |
| P5388mut4 | ATGAAAGCTTCGCTTGGCAATGGCCAGGTAACT |
| P5388mut5 | ATGAAAGCTTCGCTTGGCAATGGCCAGGTCAAT |
| P5388mut6 | ATGAAAGCTTCGCTTGGCAATGGCCAGATCGCT |
| Translational reporters | |
| 2-lacZCtermFhindcla | GCAAGCTTATTATCGATGAGCGTGGTGGTTATGC |
| 2-lacZCtermRsmakpn | CGGTACCCGGGGATCCTTATTTTTGACACCAGACC |
| YFP R HindIII | GCATCAAAGCTTATTACTTGTACAGCTCGTCCA |
| YFP F Kpn Sal | GACAGCGGTACCAATCGTCGACCATATGCTGAGCAAGGGCGAGG |
| 88-89YC-DR#2ycF | GGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCAGCATGGGGCGCTCCGGGGTTGA |
| 88-89YC-DR#2ycR | CGGCACCACGCCGGTGAACAGCTCCTCGCCCTTGCTCAGCATCGGTCTCCCCTCGTGCGG |
a
6-FAM, 6-carboxyfluorescein.