Table I.
Status of homeostatic growth control in Apc−/− 1638N COL-Pr1 cells.
| Relative to Apc+/+ C57 COL | ||
|---|---|---|
| Biomarker | Apc+/− 1638N COL | Apc−/− 1638N COL-Pr1 |
| Population doublinga | −50.0% | −64.7% |
| Saturation densityb | +2.5X | +7.6X |
| Aneuploid cell populationc | +25% | +75% |
| Aneuploid G1:S+G2/M ratioc | −54.8% | −77.4% |
| Apc target genesd | ||
| β-catenin | +4.8% | +85.7% |
| Cyclin D1 | +1.9X | +3.9X |
| c-Myc | +1.1X | +2.9X |
| COX-2 | +65.9% | +2.1X |
a
Determined from the exponential growth phase.
b
Determined at day 7 post-seeding.
c
Determined from flow cytometry-based cell cycle analysis at day 3 post-seeding.
d
Determined at day 3 post-seeding, log mean fluorescence units. Apc, adenomatous polyposis coli; COX, cyclo-oxygenase; X, fold-change.