Fig. 1.

In vitro and in vivo lentiviral transduction of hippocampal neurons with sAPPα-copGFP. a The transduction marker copGFP (green) was expressed in mouse primary hippocampal neurons co-labelled with the neuronal marker MAP2 (red) and the nuclear marker DAPI (blue). Scale bar, 50 μm. b Detection of sAPPα in media from primary mouse neural cultures transduced with LV-sAPPα, but not LV-control. The right-hand lane of the western blot illustrates the band from a sample fortified with purified sAPPα [22]. c-e Examples of in vivo copGFP expression (green) showing transduction of hippocampal regions containing LV-transduced neurons in slices from wild-type (c), transgenic-control (d) and transgenic-sAPPα (e) mice. Scale bar: 1 mm