Fig. 3.

The effect of FAR overexpression on aldehyde production. All the strains express the luciferase LuxAB, which reacts with fatty aldehydes to produce the measured luminescence signal. The S1 strain expressing Acr1, Aar or Ramo under an IPTG-inducible promoter were cultivated at 30 °C for 48 h and the luminescence signals measured. The control strains were iluxAB, which has a wild type expression of Acr1 and S1, which is FAR negative. Various IPTG concentrations were tested and the ones that yielded the highest signal are presented here. The concentrations were 1 mM for Acr1 and Aar, and 0.1 mM for Ramo. The full data with all the tested IPTG concentrations is presented in Additional file 1: Figures S2, S3. The signals are presented as averages of two independent replicates. Standard deviations were negligible and thus omitted for clarity