Fig. 4.

CBX7 regulates stem cell characteristics (GCSC phenotypes) of gastric cancer cells by activating AKT pathway. a Control and CBX7-overexpressing SGC-7901 cells were mock-treated or treated with LY294002 (50 μM) for 24 h. Western blot analysis was used to determine the expression of pAKT, AKT, CBX7, and β-actin. b–d Control and CBX7-overexpressing SGC-7901 cells were subjected to colony formation, migration, invasion, and spheroid colony formation assays in the absence or presence of LY294002. ***P < 0.001. e AKT knockdown suppresses CBX7-enhanced in vivo tumorigenicity in MGC-803 cells. In vivo tumorigenicity in CBX7, Akti, and CBX7+Akti cells was determined by subcutaneous injection of indicated gastric cancer cells into SCID mice