Fig. 1.

Sequence analysis of PtDGATX. a Phylogenetic analysis of DGATs. The PDAT gene of Chlamydomonas reinhardtii (186846) was used as the root at the outer group. Sequence alignment and phylogenetic analysis were performed with BLAST and CLUSTALW, respectively. Bootstrap support values were estimated using 100 pseudo-replicates. b The putative transmembrane construct of PtDGATX. The transmembrane region was analyzed by the online software TMHMM, DAS, and TMpred. c Topology analysis of PtDGATX via western blotting. Soluble and membrane proteins were separated and used for blotting. Actin and DGAT1 which were known soluble protein and membrane protein, respectively, were used as controls. N the N terminal, C the C terminal, Membrane transmembrane proteins, Soluble soluble proteins, PtWDoe the PtDGATX overexpression line of P. tricornutum, WT the wild-type P. tricornutum