Table 2.

Fatty acid content of TAG in the recombinant P. tricornutum

Fatty acid	Nitrogen repletion		Nitrogen depletion for 10 days
	Wild type (% of TAG)	PtWS/DGAToe (% of TAG)	Wild type (% of TAG)	PtWS/DGAToe (% of TAG)
Saturated fatty acid
C14:0	10.73 ± 0.83	11.07 ± 0.64	6.27 ± 0.23	7.36 ± 0.58
C15:0	0.59 ± 0.05	0.72 ± 0.07	0.52 ± 0.03	0.46 ± 0.04
C16:0	32.84 ± 2.45	34.47 ± 3.49	40.04 ± 3.39	43.82 ± 2.93
C18:0	7.31 ± 0.34	8.01 ± 0.48	3.07 ± 0.04	5.31 ± 0.21
C19:0	1.26 ± 0.21	1.71 ± 0.30	0.72 ± 0.01	0.84 ± 0.03
C20:0	0.31 ± 0.02	0.39 ± 0.03	0.19 ± 0.01	0.23 ± 0.01
C22:0	0.83 ± 0.07	0.81 ± 0.03	0.57 ± 0.03	0.52 ± 0.05
C24:0	3.73 ± 0.39	4.01 ± 0.23	3.24 ± 0.27	2.12 ± 0.72
Monounsaturated fatty acid
C16:1 n − 1	29.56 ± 2.32	28.06 ± 3.74	36.85 ± 2.46	34.25 ± 2.93
C16:1 n − 2	1.11 ± 0.32	0.73 ± 0.06	1.53 ± 0.17	1.20 ± 0.08
C18:1 n − 1	2.11 ± 0.34	1.71 ± 0.25	4.43 ± 0.48	2.34 ± 0.40
C18:1 n − 2	1.25 ± 0.04	1.27 ± 0.34	0.82 ± 0.02	0.17 ± 0.01
C24:1	0.27 ± 0.01	0.28 ± 0.02	0.39 ± 0.03	0.13 ± 0.01
Polyunsaturated fatty acid
C16:3	1.55 ± 0.06	1.04 ± 0.05	0.48 ± 0.03	0.53 ± 0.09
C20:3	0.33 ± 0.02	0.13 ± 0.01	0.20 ± 0.01	0.15 ± 0.01
C20:4	0.27 ± 0.01	0.21 ± 0.01	0.18 ± 0.01	0.20 ± 0.01
C20:5 (EPA)	5.86 ± 0.11	4.59 ± 0.07	0.46 ± 0.01	0.37 ± 0.01
TAG (% of DW)	3.85 ± 0.22	5.39 ± 0.15 (40%↑)	18.27 ± 1.48	22.78 ± 1.59 (25%↑)
Total lipid (% of DW)	11.25 ± 1.17	13.61 ± 1.84 (21%↑)	32.64 ± 2.51	36.18 ± 2.38 (10%↑)

DW dry weight, EPA eicosapentaenoic acid, TAG triacylglycerol, ↑ increased