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. 2018 Feb 9;11:33. doi: 10.1186/s13068-018-1038-7

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Knockdown of cel7a by the copper-responsive RNAi-mediated silencing system. Mycelial growth on double-layer agar plate containing 1% (w/v) Avicel supplied with or without copper (a). The parental and the P_tcu1–xyr1^KD strains were cultured in Avicel-induced media without copper. The total extracellular proteins from both strains were analyzed by SDS-PAGE and Western blot at the indicated time points (b)