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. 2018 Feb 9;11:33. doi: 10.1186/s13068-018-1038-7

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Redesigned culture strategy to verify the functional role of fab1 in cellulase production. Two culture procedures (a) were designed for functional study of fab1 in T. reesei using the copper-responsive RNAi-mediated silencing system. The abundance of fab1 (b) and tcu1 (c) mRNAs was quantified by qRT-PCR in the appointed sampling times. Filter paper activity was measured when mycelia were transferred into Avicel induction media at indicated time (d). Extracellular fermentation liquor (20 μL) of P_tcu1–fab1^KD strain cultured on 1% Avicel supplied with (e) or without (f) copper was analyzed by SDS-PAGE