Table 1.

Primers used in the generation of HEV Rluc replicons and their mutants

Primer IDs		Primer Sequencea	Modificationsb
Replicon
	TW4675F	5’ GCTATGAGTTCCGGGACCTCAA 3’	TW6196-Rluc
	TW5168R	5’ GGTGGCATCGCCATGCAA 3’
	TWJRhRlucU	5’ TTGCATGGCGATGCCACC ATGGCTTCCAAGGTGTACGAC 3′
	TWhRlucL	5’ AGAATTCAGCGCTGCTAGCTATTACTGCTCGTTCTTCAGCACG 3’
	SWhRluc1U	5’ TGCATCGCCCATGGGATCACCATGGCTTCCAAGGTGTACGAC 3’	pSHEV3-Rluc
	SWhRluc915L	5’ CGGTTAACATATGCTAGCCTATTACTGCTCGTTCTTCAGCACG 3’
	SWHEV4623U	5’ CGCCGAAGGAGTCTCTTAAGGGT 3’
	SWHEV5208L	5’ GTCGTACACCTTGGAAGCCATGGTGATCCCATGGGCGATGCA 3’
Mutagenic
	HEV3MvaU	5’ GTGAGGTCGGCATTTCGGATGCAATTGTCAATAACTTTTTCCTTG 3’	V1213A
	HEV3MvaL	5’ CAAGGAAAAAGTTATTGACAATTGCATCCGAAATGCCGACCTCAC 3’
	HEV3MqhU	5’ GATCAGAACCTCGGGACTCTACATGCCTTCCCGCCGTCCTGCCAG 3′	Q/H
	HEV3MqhL	5’ CTGGCAGGACGGCGGGAAGGCATGTAGAGTCCCGAGGTTCTGATC 3’
	HEV3MspU	5’ GTCTATGGGGGCCGGGCCTCATAGCCTCACTTATG 3’	S605P
	HEV3MspL	5’ CATAAGTGAGGCTATGAGGCCCGGCCCCCATAGAC 3’
	HEV3MivU	5’ GCTTGTGCCGGCTGCACTGTTAGTCCTGGGATTG 3′	I978V
	HEV3MivL	5’ CAATCCCAGGACTAACAGTGCAGCCGGCACAAGC 3’
	HEV3MvaHU	5’ GTGAGGTCGGCATTTCGGATGCTATTGTCAATAACTTTTTCCTTG 3’	V1213A (2nd)
	TWMavU	5’ TGAGGTGGGTATCTCTGATGTTATTGTTAACAACTTCTTCC 3′	A1213V TW6196
	TWMavL	5’ GGAAGAAGTTGTTAACAATAACATCAGAGATACCCACCTCA 3′
	HEV2MavU	5’ GCGAGGTGGGCATCTCCGATGTGATCGTTAATAACTTTTTCCTTG 3’	A1213V Sar55
	HEV2MavL	5’ CAAGGAAAAAGTTATTAACGATCACATCGGAGATGCCCACCTCGC 3’

^aSequences of primers

^bRluc inserted into HEV genomes or amino acid mutation introduced by the primer