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. 2018 Feb 8;19:127. doi: 10.1186/s12864-018-4499-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Dissociation, FACS separation, and RNA-seq analysis of GFP+ rod photoreceptors and GFP- retinal cells. a Experimental procedure. b Representative FACS results showing dissociated cells (inset; arrow indicates GFP+ cell), GFP+ population collected in P1, GFP- population collected in P2. Cells not in P1 or P2 are in red in the second panel. c Post-sort analysis of a sorted GFP+ population by fluorescence microscopy (inset; arrow indicates GFP+ cell) and FACS. SSC, side scatter (reflecting object complexity); FSC, forward scatter (reflecting object size)