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. 2018 Feb 9;14:13. doi: 10.1186/s13007-018-0280-0

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Rooting data and histological details of ARF in the basal region of soft shoots in walnut. a Rejuvenated soft shoots 1 day after cutting; a1 color of epidermis was yellow-white. b Rejuvenated soft shoots 5 days after cutting; b1 part of the soft shoots basal epidermis has browned. c Rejuvenated soft shoots 9 days after cutting; c1 the adventitious roots tip have broken through out the periderm. d Rejuvenated soft shoots 15 days after cutting; d1 the roots have broken through the epidermis and have assembled along the shoot. e Rejuvenated (Rj) soft shoot and mature (M) soft shoot rooting data, P < 0.05. f Mature soft shoots 20 days after cutting; f1 no ARF but some callus was evident in the basal regions. Scale bars: 10 mm (a–d, f) (a1, b1, c1, d1 was a twice magnified image respective of the white box in a, b, c and d)