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. 2018 Jan 22;62(1):2847. doi: 10.4081/ejh.2018.2847

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©Copyright K. Hashimoto et al., 2018

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Figure 8. — The panels show representative tibial chondrocyte immunostaining of MMP-3 (A-F). MMP-3 expression in the chondrocyte of WT mice at day 1, 3 and 7 after zymosan injection (A-C) at 400× magnification. MMP-3 expression in the chondrocyte of LOX-1 KO mice at day 1, 3 and 7 after zymosan injection (D-F). MMP-3 positive cells are observed both in chondrocyte of WT (A-C) and LOX-1 KO mice (D-F) during all the experimental time. MMP-3 in chondrocytes of LOX-1 KO mice (D-F) is stained weaker than in that of WT mice (AC) during all the experimental time. The graphs show the positive cell score of MMP-3 expression in the chondrocytes of WT and LOX-1 KO mice after zymosan injection at each experimental time (G). Arrows show the MMP-3 positive chondrocytes. The antibodies used were rabbit anti-mouse MMP-3 polyclonal antibody. Scale bars: 100 m.