Fig. 1.

Co-staining of wild type and genetically modified LASV GPs with Golgi and ER markers. (A), infection with monovalent VLPVs. Vero E6 cells were exposed to monovalent VLPV LJGPwt or LDGPfib and stained with anti-GP LASV antibody, or with antibody against T4fib (red), Golgi (Syntaxin 6, green), or ER (Calnexin, blue). White and yellow arrows indicate colocalization of LJGPwt and Golgi; and LJΔGPfib and ER, respectively. (B), expression of both antigens, LNΔGPfib and LJGPwt, in cells transduced with bivalent VLPV1 was detected in both compartments, in ER and in Golgi.