Skip to main content
. 2018 Feb 2;8(1):40–46. doi: 10.4314/ovj.v8i1.7

Table 1.

Primer sequences for polymorphism detection in D-loop region and on Y chromosome.

 Primer name Nucleotide sequence 5’ – 3’ Method Length
H-mtDNA F1) AGC TCC ACC ATC AAC ACC CAA A Sequencing 690 bp
H-mtDNA R1) CCA TGG ACT GAA TAA CAC CTT ATG GTT G
H-Y0147 F Biotin-ATG TCA GGA TGC CAA CTG GTC Pyroseq 74 bp
H-Y0147 R GTG CCC ACA AGA CAA TGT C
H-Y0147 S CCA CTA GGG CCC TGT TTC
H-Y0201 F Biotin-AGT GGC CCC CTG AAG ACA TT Pyroseq 76 bp
H-Y0201 R CGA CCG GAA AAG GGA CAG TG
H-Y0201 S GGC CAG AGC CAC TAC T
H-Y1277 A1 TTC TAA ACA GTA ATC TCA AAC TGT GAT KASP 57 bp
H-Y1277 A2 CTT TCT AAA CAG TAA TCT CAA ACT GTG AA
H-Y1277 C GGC TTC CAT GAA TGA CTC TCG AGT T
H-Y25345 A1 AAG TTT TAC GAA AGA CAT AAA CTA CGT TAA AT KASP 62 bp
H-Y25345 A2 GTT TTA CGA AAG ACA TAA ACT ACG TTA AAC
H-Y25345 C GAG GAT AGA TGG GGA AAA GGT TGA AAA TA
H-Y10594 F2) CCC TCT GCT GAG CAT CTA GG Sequencing 297 bp
H-Y10594 R2) TTG GAT GAA AGG GAC AGT GA
H-Y11007 F2) CCA ACA CAC GTC AAC AGC TC Sequencing 444 bp
H-Y11007 R2) GGC TTA GGC CAC TGA TGG TA

Primer sequences after:

1)

Khanshour and Cothran (2013) and

2)

Wallner et al. (2013).