Figure 2.

HA_180-195 specific hu-Ab responses and human AID expression in DRAGA mice. (A) DRAGA mice were immunized and boosted 2 weeks later with KLH-HA_180-195 conjugate. Two weeks after the boost, sera of immunized mice was assessed in ELISA plates coated with rHA of PR8 virus (2 μg/well) for titers of human anti-HA_180-195 IgM and IgG antibodies. Shown are the specific antibody titers for 3 representative mice and their corresponding signal-to-noise background of secondary antibody. (B) DRAGA mice (n = 3) were infected or not by the intranasal route with a sub-lethal dose of PR8 /A/34 virus (LD₅₀) and 7 days later the splenic RNA was extracted and analyzed by RT-qPCR using our designed primers for human AID (described in the materials and methods section). One representative DRAGA infected mouse (lanes 6 and 7) and naive DRAGA mouse (lanes 4 and 5) are shown. Control negative for human AID primers specificity was splenic RNA extracted from naive BALB/c mice (lanes 2 and 3). The CT values for duplicate samples are shown for each mouse. Arrow indicates the size of human AID amplicon.