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. Author manuscript; available in PMC: 2019 Jan 17.
Published in final edited form as: Neuron. 2018 Jan 4;97(2):356–367.e4. doi: 10.1016/j.neuron.2017.12.027
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Bacterial and Virus Strains
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Critical Commercial Assays
Deposited Data
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
C. elegans: Strain GN71: pkc-1(pg5) V Luo et al. 2014 N/A
C. elegans: Strain IK105: pkc-1(nj1) V Caenorhabditis Genetics Center WB strain: IK105 WormBase: WBvar00091271
C. elegans: Strain DCR3558 olaIs23 [Pgcy-8::caPKC-1; Pgcy-8::tagRFP; Punc-122::dsRed] This paper N/A
C. elegans: Strain DCR3055 wyIs629 [Pgcy-8::GCaMP6s; Pgcy-8::mCherry; Punc-122::GFP] This paper N/A
C. elegans: Strain DCR3245 pkc-1(pg5); wyIs629 [Pgcy-8::GCaMP6s; Pgcy-8::mCherry; Punc-122::GFP] This paper N/A
C. elegans: Strain DCR5099 olaIs23 [Pgcy-8::caPKC-1; Pgcy-8::tagRFP; Punc-122::dsRed]; wyIs629 [Pgcy-8::GCaMP6s; Pgcy-8::mCherry; Punc-122::GFP] This paper N/A
C. elegans: Strain DCR3056 olaIs17 [Pmod-1::GCaMP6s; Pttx-3::mCherry; Punc-122::dsRed] This paper N/A
C. elegans: Strain DCR3203 pkc-1(pg5); olaIs17 [Pmod-1::GCaMP6s; Pttx-3::mCherry; Punc-122::dsRed], This paper N/A
C. elegans: Strain DCR3572 olaIs23 [Pgcy-8::caPKC-1; Pgcy-8::tagRFP; Punc-122::dsRed]; olaIs17 [Pmod-1::GCaMP6s; Pttx-3::mCherry; Punc-122::dsRed] This paper N/A
C. elegans: Strain DCR3576 oyIs85 [Pceh-36::caspase-3(p12)::nz; Pceh-36::cz::caspase-3(p17); Pstrx-1::GFP; Punc-122::dsRed]; olaIs17 [Pmod-1::GCaMP6s; Pttx-3::mCherry; Punc-122::dsRed] This paper N/A
C. elegans: Strain DCR3670 olaIs17 [Pmod-1::GCaMP6s; Pttx-3::mCherry; Punc-122::dsRed]; olaIs23 [Pgcy-8::caPKC-1; Pgcy-8::tagRFP; Punc-122::dsRed]; olaEx2125 [Pgcy-8::caspase-3(p12)::nz; Pgcy-8::cz::caspase-3(p17); Pgcy-8::tagRFP; Pmyo-3::mCherry] This paper N/A
C. elegans: Strain DCR5586 olaIs70 [Pgcy-8::CX36::mCherry; Pelt-7::GFP::NLS] This paper N/A
C. elegans: Strain DCR5639 olaIs72 [Pttx-3::sl2::CX36::mCherry; Pelt-7::mCherry::NLS] This paper N/A
C. elegans: Strain DCR5793 olaIs17 [Pmod-1::GCaMP6s; Pttx-3::mCherry; Punc-122::dsRed]; olaIs70 [Pgcy-8::CX36::mCherry; Pelt-7::GFP::NLS]; olaIs72 [Pttx-3::sl2::CX36::mCherry; Pelt-7::mCherry::NLS] This paper N/A
C. elegans: Strain DCR5792 wyIs629 [Pgcy-8::GCaMP6s; Pgcy-8::mCherry; Punc-122::GFP]; olaIs70 [Pgcy-8::CX36::mCherry; Pelt-7::GFP::NLS]; olaIs72 [Pttx-3::sl2::CX36::mCherry; Pelt-7::mCherry::NLS] This paper N/A
C. elegans: Strain DCR5789 olaIs17 [Pmod-1::GCaMP6s; Pttx-3::mCherry; Punc-122::dsRed]; olaIs70 [Pgcy-8::CX36::mCherry; Pelt-7::GFP::NLS] This paper N/A
C. elegans: Strain DCR5790 olaIs17 [Pmod-1::GCaMP6s; Pttx-3::mCherry; Punc-122::dsRed]; olaIs72 [Pttx-3::sl2::CX36::mCherry; Pelt-7::mCherry::NLS] This paper N/A
C. elegans: Strain DCR5404 olaIs17 [Pmod-1::GCaMP6s; Pttx-3::mCherry; Punc-122::dsRed]; olaIs23 [Pgcy-8::caPKC-1; Pgcy-8::tagRFP; Punc-122::dsRed]; olaEx3219 [Pgcy-8::CX36::mCherry; Pttx-3::sl2::CX36::mCherry; Pmyo-3::mCherry] This paper N/A
C. elegans: Strain DCR5380 olaIs17 [Pmod-1::GCaMP6s; Pttx-3::mCherry; Punc-122::dsRed]; olaEx3219 [Pgcy-8::CX36::mCherry; Pttx-3::sl2::CX36::mCherry; Pmyo-3::mCherry] This paper N/A
Oligonucleotides
Genotyping primer: pkc1-nj1-f: att cca aaa tct gcc aaa cg This paper N/A
Genotyping primer: pkc1-nj1-r: cta aaa acc gac acc ctt gg This paper N/A
Genotyping primer: pkc1-pg5_F2: agctggcacaacttggactt This paper N/A
Genotyping primer: pkc1-pg5_R2: cgaatgctgaaaaacccaaa This paper N/A
Cloning primer F57F5.5b Forward: GGGGACAAGTTTGTACAAAAAAGCAGGCTAA ATGAGTTTTGATTGTTTGGTATACGATG This paper N/A
Cloning primer F57F5.5b Reverse: GGGGACCACTTTGTACAAGAAAGCTGGGTC GTAGGTAAAATGCGGATTGATAAATG This paper N/A
Recombinant DNA
Plasmid: DACR1040 Pgcy-8::GCaMP6s This paper N/A
Plasmid: DACR1193 Pgcy-8::caspase-3(p12)::nz Luo et al. 2014 N/A
Plasmid: DACR1194 Pgcy-8::caspase-3(p17) Luo et al. 2014 N/A
Plasmid: DACR1286 Pmod-1::GCaMP6s This paper N/A
Plasmid: DACR2397 Pttx-3::sl2::CX36::mCherry This paper N/A
Plasmid: DACR2398 Pgcy-8::CX36::mCherry This paper N/A
Plasmid: DACR2549 Pgcy-8::caPKC-1 This paper N/A
Software and Algorithms
MagatAnalyzer Gershow et al. 2012
LabView 2011 National Instruments
Metamorph Molecular Devices
ClampEx 10 Molecular Devices
ClampFit 10.7 Molecular Devices
Matlab R2016a MathWorks
Other