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Effect of nSP30 on anti-OVA IgG2a, IgG1, and IgE antibody production. Mice were i.p. injected with 100 µg of OVA with or without 3, 30, or 300 µg of nSP30 or aluminum hydroxide (Day 0). PBS or 300 µg of nSP30 alone was used as a control. On Day 21, anti-OVA IgG2a (a), IgG1 (b), and IgE (c) antibodies in sera were determined by ELISA. Values are expressed as the mean ± SEM of five mice. **P <0.01 vs. OVA/PBS (Dunnett’s test).
