Figure 4.

Effect of nSP30 on IFN-γ, IL-2, IL-4, IL-5, and IL-17 production. Mice were i.p. injected with 100 µg of OVA with or without 3, 30, or 300 µg of nSP30 or aluminum hydroxide (Day 0). PBS or 300 µg of nSP30 alone was used as a control. On Day 21, spleens were removed and splenocytes were incubated with 20, 100, or 500 µg/mL of OVA followed by measurement of IFN-γ (a), IL-2 (b), IL-4 (c), IL-5 (d), and IL-17 (e) in the culture supernatant. Values are shown as the mean ± SEM of triplicate samples from culture supernatants of cell pooled from five mice. *P <0.05, **P <0.01 vs. OVA/PBS (Dunnett’s test).