Figure 1.

Protective effect of XN mycelia extracts on % PC 12 cells against H₂O₂ induced cell damage as analyzed by (a) MTT assay, (b) lactate dehydrogenase (LDH) release assay, flow cytometric assay for (c) the percentage of PC12 cells apoptosis, and (d) the mitochondrial membrane potential (MMP, Δψ) depolarization (%). Cell viability was determined by measuring MTT reduction and LDH release activity was measured using a colorimetric LDH assay kit. For apoptosis detection, cells were labelled with a combination of annexin V-FITC/PI and measured by flow cytometry, acquiring 10,000 gated events per fraction. For MMP (Δψ), cells were stained with JC-1, washed twice with 1× assay buffer and measured by flow cytometry, acquiring 20,000 gated events per fraction. Data are presented as the means ± SD (n = 3). A, B, and C indicate values with significant differences (P < 0.05) compared to controls, as analyzed by Duncan’s multiple range test.