Figure 2.

Comet assay analysis of the prevention of H₂O₂-induced DNA damage in PC12 cells by XN mycelia extracts. (a) Untreated control; (b) H₂O₂ treated; (c) hot water extract (10 µg/mL) + H₂O₂; (d) hot water extract (50 µg/mL) + H₂O₂; (e) hot water extract (100 µg/mL) + H₂O₂; (f) ethanol extract (10 µg/mL) + H₂O₂; (g) ethanol extract (50 µg/mL) + H₂O₂; (h) ethanol extract (100 µg/mL) + H₂O₂; (i) tail length graph and (j) tail DNA percentage graph of Comet Assay. DNA damage was determined by comet assay and data analyzed using the OpenComet software tool in image J software. The data are presented as the mean ± SD (n = 3). A, B, and C represent tail length (µm) and a, b, c represent % tail DNA with significant differences (P < 0.05) compared to controls, as analyzed by Duncan’s multiple range test.