Figure 10.
Western blot for PDE4D. Mast cells (HLMC, 0.8 × 106 cell equivalents) and basophils (BAS, 0.8–1 × 106 cell equivalents) were solubilized and along with recombinant PDED1 (r-PDE4D) subjected to SDS-PAGE. The proteins were then transferred electrophoretically to nitrocellulose membranes and membranes probed with monoclonal antibody to PDE4D. Molecular weights (kDa) of standards (S) are indicated. The recombinant protein has a predicted molecular weight of 67 kDa but runs at 72 kDa. Results are representative of a total of six experiments. Mast cell and basophil purities were 99% and 92%, respectively.