Figure 14.
Western blot for PDE7. Mast cells (HLMC, 0.5 × 106 cell equivalents) and basophils (BAS, 0.5 × 106 cell equivalents) and Hut78 cells were solubilized and along with recombinant PDE7A2 (r-PDE7A) subjected to SDS-PAGE. The proteins were then transferred electrophoretically to nitrocellulose membranes and membranes probed with monoclonal antibody to PDE7A2. Molecular weights (kDa) of standards (S) are indicated. The recombinant protein PDE7A2 has a calculated molecular weight of 55 kDa. The blot is representative of a total three of similar design. Mast cell and basophil purities were 99% and 92%, respectively.