Figure 2.
Western blot for PDE4A. Mast cells (HLMC, 0.8 × 106 cell equivalents) and basophils (BAS, 0.5 × 106 cell equivalents) were solubilized and along with recombinant r-PDE4A subjected to SDS-PAGE. The proteins were then transferred electrophoretically to nitrocellulose membranes and membranes probed with monoclonal antibody to PDE4A. Molecular weights (kDa) of standards (S) are indicated. The recombinant protein PDE4A has a calculated molecular weight of 98 kDa. Results are representative of a total of six experiments. Mast cell and basophil purities were 99% and 92%, respectively.