Figure 5.

Determination of message for PDE4C. RNA was extracted from mast cells (HLMC), basophils (BAS), and mononuclear cells (MNC) and reverse transcribed in the absence (-) or presence (+) of MMLV-RT. Amplification of cDNAs and genomic DNA isolated from lung tissue (LT) was performed using primers for PDE4C and a water blank was also subjected to the amplification step. PCR products and a 100 bp ladder, serving as molecular weight standards (S), were then subjected to electrophoresis and bands visualized with ethidium bromide. The expected molecular weight of the amplified product is 336 bp. Results are representative of four or five experiments. Mast cell and basophil purities were 99% and 92%, respectively.