Figure 9.

Determination of message for PDE4D1. RNA was extracted from mast cells (HLMC), basophils (BAS), and mononuclear cells (MNC) and reverse transcribed in the absence (-) or presence (+) of MMLV-RT. Amplification of cDNAs was performed using primers for PDE4D1 and a water blank was also subjected to the amplification step. PCR products and a 100 bp ladder, serving as molecular weight standards (S), were then subjected to electrophoresis and bands visualized with ethidium bromide. The expected molecular weight of the amplified product is 530 bp. Results are representative of a total of six experiments. Mast cell and basophil purities were 99% and 92%, respectively.