Table 1. Primers used for qRT-PCR array validation.
Gene | Gene name | Accession number | Temp | size (bp) | Primer |
---|---|---|---|---|---|
Atf3 | activating transcription factor 3 | NM_012912 | 60°C | 233 | F: CCAGAACAAGCACCTTTGCC R: CGGCATTCACACTCTCCAGT |
Ecel1 | Endothelin converting enzyme-like 1 | NM_021776 | 60°C | 134 | F: TCTCGACATGCGTGAGATCG R: ACAACAGTCGGTTGAGGTCC |
GFAP | glial fibrillary acidic protein | NM_017009 | 60°C | 143 | F: GAAATTGCTGGAGGGCGAAG R: TCTCCACCGTCTTTACCACG |
Timp1 | TIMP metallopeptidase inhibitor 1 | NM_053819 | 60°C | 140 | F: CGCTAGAGCAGATACCACGA R: CCAGGTCCGAGTTGCAGAAA |
Tp53 | Tumor protein p53 | NM_030989 | 60°C | 217 | F: CTGGACGACAGGCAGACTTT R: GACAGGCACAAACACGAACC |