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. 2018 Feb 9;13(2):e0192530. doi: 10.1371/journal.pone.0192530

Table 1. Summary quantitative image quality and illumination metrics and test targets.

Equation(#) Metric Formula Test Target
1 Minimal Resolvable Feature Size (micron) 10002(Group#+1+Element#16),
Each group of Ronchi rulings consists of six elements, numbered from 1 to 6 (large to small physical size). The group and element of the smallest discernable set will provide resolvable feature size.
USAF 1951
(#R3L3S1P;Thorlabs Inc., Newton NJ)
2 Diagonal Field of View (mm) [(Total#ofHorizontalDetectorHorizontalLineLength)*(2.5mm2(Group#+Element#16))]2+[(Total#ofVerticalDetectorVerticalLineLength)*(2.5mm2(Group#+Element#16))]2,
The Total # of Pixels in the Horizontal Detector axis = 2592, the Total # of Pixels in the Vertical Detector axis = 1944, the Horizontal Line Length = width in pixels of the selected vertical Ronchi ruling, the Vertical Line Length = width in pixels of the selected horizontal Ronchi ruling. The Group and Element correspond to the selected Ronchi ruling.
3 Depth of Focus (mm) A 45° angled face resolution target with horizontal and vertical lines at a frequency of 5 line pairs per mm, corresponding to a feature size of 31.25 μm. 5-15DOF
(#54–440, Edmund Optics, Barrington NJ)
4 Standard Mobile Imaging Architecture (SMIA) Television Distortion (%) (AB)B*100% and A=A1A22, where A1 and A2 are the outer side lengths of a square and B is the distance between the midpoints of the sides of the square A1 and A2. “+” = pincushion distortion and
“-” = barrel distortion
SFRplus
#CoG (imatest LLC., Boulder CO)
5a Color Reproduction Error with Luminance Difference ΔEab*=((ΔL*)2+(Δa*)2+(Δb*)2), L* for lightness (or luminosity in the z axis), and x- and y-axis are cube root transforms of color data in a* and b*, color-opponent dimensions X-Rite Rez Checker
(#87–422; Edmund Optics, Barrington NJ)
5b Color Reproduction Error ΔCab*=((Δa*)2+(Δb*)2), x- and y-axis are cube root transforms of color data in a* and b*, color-opponent dimensions
6 Percentage Specular Reflection #ofsaturatedpixelstotal#ofpixels*100%, where a saturated pixel defined as pixel intensity ≥ 250. #s504 Zoe
(Gaumard Scientific, Miami FL)
7 Weber’s Contrast Ratio CW=IBIFIB, IB = Background Pixel Intensity
IF = Foreground Pixel Intensity
8 Thermal Safety IEC 60601–1 temperature limit of <48°C after 1 hour continuous operation N/A

Minimal threshold used for our study was CW ≥ 0.5.