Fig 7. Inactivation of Rap1 rescues the Rasa3-depleted phenotypes.
A. Quantification of capillary-like network formation in siControl vs siRasa3 HUVEC treated or not with GGTI298 (left) or co-transfected with siRap1A, siRap1B or a second control siRNA (right). Results are expressed as mean ± SD cumulative length of capillary-like structures measured in 5 different fields per experiment from 3 independent experiments, relative to non-treated siControl HUVECs (left) or to double siControl HUVECs (right), respectively. B. Number of sprouts growth out from R3f/f and R3f/f iEC-Cre aortic rings after 8 days of culture in the presence or absence of the Rap1 inhibitor GGTI298. A minimum of 10 aortic rings were analyzed per group in 3 independent experiments. C. Representative orthogonal reconstruction images of confocal Z-stacks from R3f/f and R3f/f iEC-Cre aortic ring sprouts, in the presence or absence of the Rap1 inhibitor GGTI298, stained for the IB4 (green) endothelial and nuclei DAPI (blue). Lumens are outlined with a white dotted line. Bars = 5 μm. Graph represent the mean ± SEM of the percentage of lumenized sprouts from R3f/f and R3f/f iEC-Cre aortic rings cultured in the same conditions as in panel B. The p values are shown (Student’s t-test).