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. Author manuscript; available in PMC: 2018 Dec 26.
Published in final edited form as: Biochemistry. 2017 Dec 14;56(51):6629–6638. doi: 10.1021/acs.biochem.7b01170

Figure 2.

Figure 2

SHAPE-Seq characterization of the agsA thermometer in vivo. (a) SHAPE-Seq normalized reactivity (ϱ) plots for agsA WT, G21C, and A29C variants from triplicate experiments in vivo performed at 30 °C and using 42 °C heat shock conditions. Error bars represent standard deviations over replicates, and stars represent statistical significance between 30 °C and 42 °C reactivities. (b) Experimentally-informed minimum free energy (MFE) RNA structures as predicted using the RNAStructure Fold program with the reactivity values from (a) taken as pseudoenergy folding restraints.29 Nucleotides are color-coded according to normalized SHAPE reactivity (ϱ), with red indicating high reactivity and black low reactivity. Point-mutated nucleotides are shaded in grey.