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. Author manuscript; available in PMC: 2018 Dec 26.
Published in final edited form as: Biochemistry. 2017 Dec 14;56(51):6629–6638. doi: 10.1021/acs.biochem.7b01170

Figure 4.

Figure 4

Testing agsA thermometer function in a cell-free protein synthesis system using purified pre-transcribed mRNA. (a) Fluorescence trajectories over time for translation of SFGFP from agsA constructs and control mRNA in the PURExpress protein synthesis system at 30 °C and 42 °C. Shading represents standard deviation over three replicates. (b) SFGFP production rates, calculated from the trajectories in (a), during the linear synthesis regime for agsA constructs and control at 30 °C (45–50 minutes) and 42 °C (30–35 minutes), with error bars representing standard deviation.