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. Author manuscript; available in PMC: 2018 Dec 26.
Published in final edited form as: Biochemistry. 2017 Dec 14;56(51):6629–6638. doi: 10.1021/acs.biochem.7b01170

Figure 6.

Figure 6

Investigating the effect of translation and ribosome occupancy on agsA WT structure and reactivity. (a) agsA WT SHAPE-Seq reactivity plot for triplicate experiments carried out in vitro in PURExpress without ribosomes. (b) agsA WT SHAPE-Seq reactivity plot in PURExpress with active translation and difference plot of reactivities showing the change from the no ribosome case in part (a). (c) agsA WT SHAPE-Seq reactivity plot in PURExpress with stalled translation initiation complexes generated by supplying only methionine and difference plot of reactivities showing the change from the no ribosome case in part (a). Error bars represent standard deviations over replicates. Stars represent statistical significance as in Figure 5.